Leica-biosystems ANGLE TWO User Manual

Living up to LifeLeica Angle Two™Computer-Assisted Stereotaxic SystemUser Manual

ComponentsLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 103.1 U FrameFigure 4 shows the Angle Two U frame with a rat ada

ComponentsLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 11Caution Do not use the rat incisor bar on a mouse. A mouse ada

ComponentsLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 12The ear bars are locked into position using the locking levers

ComponentsLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 133.2 Manipulator: Linear MovementsThe manipulator is attached t

ComponentsLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 14Each linear movement is controlled by a coarse drive and, in t

ComponentsLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 15Fine DriveThe fine drive (see Figure 9) provides greater contr

ComponentsLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 163.3 Manipulator: Tilt, Rotation and Swing-OutTilt, rotation an

ComponentsLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 17TiltThe primary tilt plane can be in either the AP or ML direc

ComponentsLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 182. Unlock the rotation clamp and rotate the base of the manipu

ComponentsLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 19Swing-Out Use swing-out to change the primary tilt plane by 90

Leica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 2Intended Use StatementThe Angle Two™ system is designed for stereotaxic s

ComponentsLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 203.4 Probe ShaftA clamp attaches the probe shaft to the manipul

ComponentsLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 21Figure 20. The probe shaft screwAttaching a ProbeAt the botto

ComponentsLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 223.5 Vernier ScalesAll Leica stereotaxic instruments have verni

ComponentsLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 233.6 Computer The computer consists of a desktop case, monitor,

Leica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 244Software Overview This chapter describes the Angle Two™ software and it

Software OverviewLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 254.1 AtlasThe instrument comes with either one mouse or

Software OverviewLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 264.2 ModesFour modes are used during surgery: Set Target

Software OverviewLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 273. Manually enter the coordinates into the Target (Red)

Software OverviewLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 28AtlasThe Atlas mode shows the exact position of the pro

Software OverviewLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 294.3 Initial SettingsBefore locating the target site, yo

Leica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 3ContentsLeica Angle Two™ . . . . . . . . . . . . . . . . . . . . . . . .

Software OverviewLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 30Enter OffsetsOffsets are used when performing surgery o

Software OverviewLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 314.4 CoordinatesCoordinates can be saved or exported at

Software OverviewLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 321. Enter a name and description.2. In Choose Source Poi

Leica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 335AlignmentAlign the instrument whenever the software is started.Alignmen

AlignmentLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 345.2 ProcedureThe primary tilt of the instrument can be in eithe

AlignmentLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 355. Attach the clamp adaptor to the dial indicator by sliding th

AlignmentLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 368. Attach the other end of the alignment shaft into the manipul

AlignmentLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 37Horizontal AlignmentThe ML horizontal path of the probe is cont

AlignmentLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 3812. Use the ML drive to move the spring horizontally along the

AlignmentLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 39AP Tilt13. If the tilt is in the AP direction use the ML drive

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AlignmentLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 4015. When the AP tilt is aligned, select ll To AP on the compute

AlignmentLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 4114. Move the spring to the bottom and then up using the DV driv

Leica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 426Using the InstrumentThis chapter describes the whole process of using t

Using the InstrumentLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 43Preparation1. Ensure that the instrument is clean (s

Using the InstrumentLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 4411. Support the head and insert the right ear bar in

Using the InstrumentLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 45Locating the Target Site22. Click To Target (see “To

Using the InstrumentLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 46Probe Placement30. Unlock the swing-out clamp and re

Leica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 477Cleaning and MaintenanceCaution Do not autoclave. The bushings and prob

Leica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 488SpecificationsInstrument DimensionsResolution, Linear ScalesDrive Advan

SpecificationsLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 49Tooth Bar Movement RangesProbe HolderComputerMonitorDV 20

Leica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 51IntroductionThe Leica Angle Two™ stereotaxic system is designed to perfo

Software Licence TermsLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 50Software Licence Terms The following License Terms

Leica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 62Instrument InstallationThe Leica Angle Two™ system needs to be assembled

Instrument InstallationLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 72.2 AssemblyWhen unpacked the Angle Two instrument

Instrument InstallationLeica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 82. Connect the five instrument cables into the por

Leica Angle Two™ User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 93ComponentsThis chapter describes the parts of the Leica Angle Two™ syste