Leica-biosystems SP2600 User Manual

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Leica SP2600
Ultramilling attachment
Supplementary instruction manual Leica SP2600
To be used only in conjunction
with instruction manual Leica SM2500 .
V1.2 English  07/2002
Always keep this manual near the instrument.
Read carefully prior to operating the instrument.
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Summary of Contents

Page 1 - Leica SP2600

Leica SP2600Ultramilling attachmentSupplementary instruction manual Leica SP2600To be used only in conjunctionwith instruction manual Leica SM2500 .V1

Page 2

10Supplementary instruction manual V 1.2  07/2002Be sure to read and comply with thesafety instructions, warnings and cau-tions in this cha

Page 3

11Leica SP2600  Ultramilling attachmentSafety instructions - transport and installationCaution!Only the vacuum cleaner offered byLeica or other

Page 4 - Table of contents

12Supplementary instruction manual V 1.2  07/2002Safety instructions -cleaning disinfection and maintenanceOnly authorized service techniciansmay

Page 5 - Important information

13Leica SP 2600  Ultramilling attachment3. Technical DataUltramilling attachmentMilling spindle (special design)...

Page 6 - 1. Important information

14Supplementary instruction manual V 1.2  07/20024. Installation4.1 Overview - Leica SP2600 Ultramilling attachment / basic instrument Leica SM2500-

Page 7

15Leica SP2600  Ultramilling attachment4. Installation4.2 Leica SP2600 standard deliveryStandard delivery includes:1 Basic instrument Leica SP2600

Page 8

4.5 Installing the Leica SP2600 ultramilling attachment Place the carrier (1, Fig. 4.2) for the ultramilling attachment (3, Fig. 4.3) onthe rear part

Page 9

4. Installation4.6 Connecting the vacuum cleanerMilling dust is extracted by a vacuum cleaner, through a nozzle (1) located inthe milling area.230 V /

Page 10 - 2. Safety

18Supplementary instruction manual V 1.2  07/20024.7 Installing additional accessories Install further accessories as described in chapters 4.5.2 an

Page 11

19Leica SP 2600  Ultramilling attachment5.1 Overview of chapter 5 OperationPrior to working with the Leica SP2600 ultramilling attachment, besure t

Page 13 - 3. Technical Data

20Supplementary instruction manual V 1.2  07/20025.2 Adjusting the pre-millers in thecalibrating devicePreparatory steps Connect mains power supply

Page 14 - 4. Installation

21Leica SP2600  Ultramilling attachmentAdjusting the millerThe miller (3) is adjusted in the holder (2) alongthree different axis (x, y and z - see

Page 15

22Supplementary instruction manual V 1.2  07/2002Adjustment along the z-axis1. Pull lever (12) at the left of calibration device(18) forward as far a

Page 16

23Leica SP2600  Ultramilling attachment6. Insert the size-5 Allen key into regulatingscrew (4) insert size-2.5 Allen key into regu-lating screw (11

Page 17

24Supplementary instruction manual V 1.2  07/2002Adjustment along the y-axis1. Select reference value 1.The reference value will be neededlate

Page 18

25Leica SP2600  Ultramilling attachment5.3 Adjusting the finishing millers in thecalibrating device Carry out preparatory steps as per chapter 5.2,

Page 19 - 5. Operation

26Supplementary instruction manual V 1.2  07/2002Adjusting the finishing millerJust like the pre-miller, the finishing miller is ad-justed in the mil

Page 20

27Leica SP2600  Ultramilling attachmentFig. 5.12 411376 Insert size-5 Allen key into regulating screw(4) insert size-2.5 Allen key into regulatingsc

Page 21

28Supplementary instruction manual V 1.2  07/2002Adjustment along the y-axis1 Select the same reference value as was setfor the pre-miller.Pre-miller

Page 22

29Leica SP 2600  Ultramilling attachment5.4 Specimen holder systems - how to combine individual components5. OperationConfigurations:1. Base plate wi

Page 23

3Leica SP2600  Ultramilling attachmentThe information, numerical data, notes and valuejudgments contained in this manual represent thecurrent state

Page 24

30Supplementary instruction manual V 1.2  07/20025.5 Clamping specimenFor a detailed overview of the clamping systems available, pleaserefer to chapt

Page 25

31Leica SP2600  Ultramilling attachment5.5.3 Clamping the vacuum stages on/inthe base platesClamping the vacuum stages in the base platewith vise (F

Page 26

32Supplementary instruction manual V 1.2  07/20025. Operation5.5.4 Connecting the vacuum pump /placing specimens onto the vacuumstagesIMPORTANT:Prior

Page 27

33Leica SP2600  Ultramilling attachmentPlacing specimens onto vacuum stage for 30 -50-mm lids Fasten a lid suited to the size of the specimento be

Page 28

34Supplementary instruction manual V 1.2  07/20025.6 Preparatory steps prior to the milling procedureFor a detailed description of all control e

Page 29

35Leica SP 2600  Ultramilling attachment Insert the specimen (see chapters 5.5.2 and 5.5.4, pages 30 and 33 of thismanual) or - if you need to mill

Page 30

36Supplementary instruction manual V 1.2  07/20025.7 Pre-milling Select automatic mode of operation AUTO (button (5) - chapter 5.4.3, page26 Leica

Page 31

37Leica SP 2600  Ultramilling attachmentRecommended milling thickness settings for pre-milling 10 µm - keep milling until surface is completely leve

Page 32

38Supplementary instruction manual V 1.2  07/20025.7.1 Perspective view of the pre-milling procedure5. OperationPre-milling procedureThe milling spin

Page 33

39Leica SP 2600  Ultramilling attachment5. OperationEnding the pre-milling procedure Press RUN STOP or RUN ENABLE to stop the sledge (buttons (29),

Page 34

4Supplementary instruction manual V 1.2  07/2002Table of contents1. Important information...

Page 35

40Supplementary instruction manual V 1.2  07/20025.8 Finishing milling Attach the miller holder containing the finishing miller to the millingspindl

Page 36

41Leica SP 2600  Ultramilling attachmentRecommended sledge speed settings for finishing milling Milling stroke: 0.5 mm/s to 1.0 mm/s Return stroke:

Page 37

42Supplementary instruction manual V 1.2  07/20025.8.1 Perspective view of the finishing milling procedure5. OperationFinishing milling procedureThe

Page 38

43Leica SP 2600  Ultramilling attachmentEnding the finishing milling procedure Press RUN STOP or RUN ENABLE to stop the sledge (buttons (29), (30) -

Page 39

44Supplementary instruction manual V 1.2  07/20026. Troubleshooting6. TroubleshootingFor all instructions on error detection and trouble shooting, pl

Page 40

45Leica SP 2600  Ultramilling attachment7. Cleaning, disinfection and maintenance7.1 Cleaning and disinfectionOnly authorized service technicians may

Page 41

46Supplementary instruction manual V 1.2  07/20027. Cleaning, disinfection and maintenance7.2 MaintenanceOnly authorized service technicians may acce

Page 42

47Leica SP 2600  Ultramilling attachment7.2.2 Replacing the light bulb in the calibration device Remove base cover (2) of calibration device (1) (un

Page 43

48Supplementary instruction manual V 1.2  07/20028. Overview - accessories8.1 Miller holders and millersMiller holderHolder for pre- and finishing mi

Page 44 - 6. Troubleshooting

49Leica SP 2600  Ultramilling attachment8.5 Vacuum stages and matching lids / perforated specimen holders Vacuum stage for Leica SP2600 - basic body

Page 45 - 7.1 Cleaning and disinfection

5Leica SP 2600  Ultramilling attachmentImportant information6. Troubleshooting ...

Page 46 - 7.2 Maintenance

50Supplementary instruction manual V 1.2  07/20028.7 Additional accessoriesVacuum cleaner and vacuum cleaner accessories Vacuum cleaner Fakir S20

Page 47

51Leica SP 2600  Ultramilling attachment9. Warranty and serviceFor all information on warranty and service, please refer to chapter 9, pages74 - 76 o

Page 48 - 8. Overview - accessories

6Supplementary instruction manual V 1.2  07/20021. Important information1.2 Designated useThe Leica SP2600 is designated to prepare milledsurfaces an

Page 49

7Leica SP 2600  Ultramilling attachment1. Important information1.3 Information on this instruction manualInstrument typeThis supplementary instructio

Page 50 - 8 Overview - accessories

8Supplementary instruction manual V 1.2  07/20022. Safety2.1 Safety regulations This instrument has been built and tested in accordance with the same

Page 51 - 9. Warranty and service

9Leica SP 2600  Ultramilling attachment2. Safety2.2 Built-in safety devicesThe instrument is equipped with the following safety devices:Plexiglass pr

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