Leica-biosystems ST4040 User Manual

Leica ST4040Linear StainerInstruction ManualLeica ST4040 V1.4 - Rev A, English – 06/2009Always keep this manual near the instrument.Read carefully pr

10Instruction Manual V1.4 – 06/20092.1.3 Cleaning and maintenance• Only technical service engineers authorizedby Leica may access the internal compo-

11Leica ST4040 – Linear StainerDo not operate the instrument in roomswhere risk of explosion exists!Do not expose the instrument to directsunlight (wi

12Instruction Manual V1.4 – 06/20093. Installation3.2 Unpacking the instrumentThe unpacking instructions for all Leica ins-truments are located in a

13Leica ST4040 – Linear Stainer3.3 Installing the instrument• To lift, grip the instrument by the transporthandles.Two people are needed to lift / car

14Instruction Manual V 1.4 – 06/20093. Installation3.4 Standard delivery ‘basic instrument’ and general accessories3.4.1 Standard deliveryBasic instru

15Leica ST4040 – Linear Stainer3. Installation3.4.2 General accessories• Reagent stations, plastic• Tap water stations, complete assy. with inlet valv

16Instruction Manual V1.4 – 06/20093.5 Electrical connections3.5.1 Adjusting the voltage selectorThe voltage selector is preset to matchthe nominal v

17Leica ST4040 – Linear Stainer3.6.2 Installing the drain hose• Connect the drain hose.Upon installing the drain hose makesure there is a gradient fro

18Instruction Manual V1.4 – 06/20093.6.6 Leveling the instrumentWhen installed in its final posi-tion onthe laboratory bench, the instrumentmust be e

19Leica ST4040 – Linear Stainer3.6.7 Inserting the activated carbon filterIf the instrument is not connected to afume hood (via exhaust air hose), ana

20Instruction Manual V1.4 – 06/20094.1 Site requirementsThe installation site has to meet the followingrequirements:• Stable laboratory bench, exactl

21Leica ST4040 – Linear Stainer4. Installation of the Optional Load/Unload Station4.3 Standard delivery and accessories - optional load / unload stati

22Instruction Manual V1.4 – 06/20094.4 Installation4.4.1 Installing the optional load station• Remove the 4 hexagon socket screws fromthe left lid (h

23Leica ST4040 – Linear Stainer• Place the optional load station on the leftside of the basic instrument.• Pull the conveyor frame of the optional loa

24Instruction Manual V1.4 – 06/20094. Installation of the Optional Load/Unload Station• Fit the connecting plates attached to thefront and rear half

25Leica ST4040 – Linear Stainer4.4.2 Installing the optional unload stationon the right of the basic instrument• Remove the 4 hexagon socket screws fr

26Instruction Manual V1.4 – 06/2009• Pull the conveyor frame of the optional un-load station upwards using both your hands.• Fit the connecting plate

27Leica ST4040 – Linear Stainer• Use the 4 hexagon socket screws which youhave kept (see step 1) to attach the lid of theoptional unload station to th

28Instruction Manual V1.4 – 06/20094. Installation of the Optional Load/Unload Station• Place grooved ramp no. 2 onto the rear pinand use the hexagon

29Leica ST4040 – Linear Stainer5.1 Technical DataGeneralAdmissions: VDE, UL, cUL, C-Tick LabelNominal supply voltage:

3Leica ST4040 – Linear StainerIssued by:Leica Biosystems Nussloch GmbHHeidelberger Str. 17 - 19D-69226 NusslochGermanyPhone: +49 6224 143-0Fax: +49

30Instruction Manual V 1.4 – 06/20096.2 Preparing the instrument for staining: inserting and filling the stations• Select a staining protocol.(For exa

31Leica ST4040 – Linear Stainer6. Operation6.3 Staining in two rows6.3.1 Doubling the specimen throughput• For this type of application, the sequences

32Instruction Manual V 1.4 – 06/20096.4 Control panel functions6. OperationIMMERSION TIMEbutton -Display / select im-mersion timeDRAINING TIMEbutton -

33Leica ST4040 – Linear Stainer6. Operation6.5 Switching on mains and selecting direction of travel and alarm volume• Switch on mains (O = OFF / I = O

34Instruction Manual V 1.4 – 06/20096.5.2 Selecting alarm volume• Press the ‘Immersion time’ button and hold.• Press ‘+’ or ‘-’ respectively:• Each ti

35Leica ST4040 – Linear Stainer6.7 ProgrammingProgramming must be done while in stand-by mode. The instrumentcan store 2 programs: (buttons ‘Prog 1’ a

36Instruction Manual V 1.4 – 06/20096. Operation• Draining time can be set from 0 seconds to 60 seconds.• Press the agitation button to activate or de

37Leica ST4040 – Linear Stainer6.8.2 Preparing and inserting the slide racks• Insert the slides into the slide rack/s.• Attach the slide rack carrier

38Instruction Manual V 1.4 – 06/20096.8.3 Starting the selected program• With the lid closed, open the ball valve and, if closed as well, open thewate

39Leica ST4040 – Linear Stainer6.9 Removing the slide racksAs soon as the alarm is triggered, immediately remove the completedslide rack from the last

4Instruction Manual V 1.4 – 06/2009Table of ContentsIMPORTANT NOTE ...

40Instruction Manual V 1.4 – 06/20096.10 Removing the slide racks in instruments equipped with optional unload station• To remove the slide racks, pro

41Leica ST4040 – Linear Stainer6. Operation6.11 Finishing work• Once the last slide rack is removed, press ‘Start/Stop’.•Close the ball valve.Do not c

42Instruction Manual V1.4 – 06/2009Possible causeProblem What to do- Open water tap.- Open ball valve.- Call Technical Service.- Call in-house techni

43Leica ST4040 – Linear StainerPossible causeProblemWhat to do7. Trouble Shooting- Clean drain hose (seechapter 8 - ‘Cleaning /Main-tenance’) - If nec

44Instruction Manual V 1.4 – 06/20098.1 CleaningPrior to cleaning the instrument, always switch off mains and unplugthe power chord!Dispose of used re

45Leica ST4040 – Linear Stainer8. Cleaning and Maintenance8.1 Cleaning• Clean the reagent and tap water stations as well as the trough whichholds the

46Instruction Manual V 1.4 – 06/20098.2 MaintenanceOnly authorized Leica technical service engineers may open the in-strument for maintenance and repa

47Leica ST4040 – Linear StainerWarrantyLeica Biosystems Nussloch GmbH guarantees that the contractual prod-uct delivered has been subjected to a compr

48Instruction Manual V 1.4 – 06/2009We herewith declare, in exclusive responsibility, that theLeica ST4040 – Linear stainerwas developed, designed and

49Leica ST4040 – Linear StainerAppendix 1Staining Protocols for Leica ST4040 Linear StainerXyleneXyleneXyleneXylene100 % Alcohol100 % Alcohol96 % Alco

5Leica ST4040 – Linear StainerTable of ContentsAccessories - optional unload station ...

50Instruction Manual V 1.4 – 06/2009Ordering InformationLid, cpl. w/handle 14 0474 32255Slide rack carrier Leica 14 0474 32305Slide rack carrier Medit

6Instruction Manual V1.4 – 06/2009The instruction manual for the LeicaST4040 includes chapters on the followingsubjects:Chapter 1 Structure of this

7Leica ST4040 – Linear StainerGeneralThis instruction manual includes important in-structions and information related to the operat-ing safety and mai

8Instruction Manual V1.4 – 06/20092.1.1 Transport and installation• Do not operate the instrument in roomswhere risk of explosion exists!• Do not exp

9Leica ST4040 – Linear Stainer2. Safety2.1.2 Operating the instrument• The instrument may only be operated byskilled personnel. It may only be operate