Leica-biosystems CM1860/CM1860 UV User Manual

Instructions for UseLeica CM1860/CM1860 UV, V1.4 – 10/2012, EnglishOrder No.: 14 0491 80101 RevEAlways keep this manual with the instrument.Read caref

10Instructions for Use V 1.4 – 10/20122. SafetyUVC disinfection is effective when disinfecting sur-faces and air within the irradiated working area of

11Leica CM1860 /CM1860UV – CryostatMedium disinfection can be attained through short irradiation for 30 minutes (CM1850UV/CM1860UV/CM1950). This reduc

12Instructions for Use V 1.4 – 10/20122.3.2 Knife guardPrior to manipulating the knife and specimen, changing the speci-men or knife, or taking a bre

13Leica CM1860 /CM1860UV – Cryostat2. Safety2.4 Operating conditionsTransport and installation• Aftertransporting,donotturntheinstrumenton

14Instructions for Use V 1.4 – 10/20122.5 Operating the instrument2. Safety2.6 Cleaning and disinfection• Takecarewhen handlingmicrotomeknives

15Leica CM1860 /CM1860UV – Cryostat2.7 Removing the microtome• Themicrotomeisencapsulatedandthereforedoesnotrequireremovalby the user.2.8

16Instructions for Use V 1.4 – 10/20123. Technical DataOperating temperature range (ambient temperature): 18 °C to 35 °C. All specifications related

17Leica CM1860/CM1860 UV – CryostatObserve item 4.1, "Installation site requirements".3. Technical DataMicrotomeRotary microtome Secti

18Instructions for Use V 1.4 – 10/20124.1 Installation site requirements4. Setting up the InstrumentDo not operate in rooms with explosion hazard! T

19Leica CM1860 /CM1860UV – CryostatWhen the instrument is delivered, check the tilt indicators on the packaging.If the arrowhead is blue, the shipment

20Instructions for Use V 1.4 – 10/20124. Setting up the Instrument• Liftandremovethewoodenedge(6).• Removetheramp(5) on the front side of

21Leica CM1860 /CM1860UV – Cryostat• Whentransportingtheinstrumentonwheels,(2) grip the cabinet only at the marked loca-tions ( ).• Toensure

22Instructions for Use V 1.4 – 10/20124.3 Standard delivery—packing listBasic instrument in the respective voltage variant:1 knob with marking, anti

23Leica CM1860/CM1860 UV – Cryostat4.4 Assembling the handwheelThe handwheel and all parts for installation are included in the carton with the acces

24Instructions for Use V 1.4 – 10/20125.1 Electrical connection5. Instrument SetupDuring the start-up of the refrigeration system, the minimum nomin

25Leica CM1860/CM1860 UV – Cryostat5. Instrument Setup5.3 Overview of the Leica CM1860/CM1860UV416151718192013129118101314Fig. 111321

26Instructions for Use V 1.4 – 10/2012 1 Cryostat CM1860/ CM1860 UV 2 Waste container 3 Control panel 1 4 Control panel field 2 5 Storage she

27Leica CM1860 /CM1860UV – Cryostat5.4 Power switch and circuit breaker The switch must be in the top position (switch position 1) for switching on

28Instructions for Use V 1.4 – 10/20126. Operating the Instrument6.1 Control panel 1Fig. 14Function keysLamp buttonON/OFF switch for cryochamber ill

29Leica CM1860/CM1860 UV – CryostatTo start disinfection, the sliding window must be completely closed.Press the UVC button once briefly, 30 min. mode

3Leica CM1860/CM1860UV – CryostatPublished by:Leica Biosystems Nussloch GmbHHeidelberger Str. 17 - 19D-69226 NusslochGermanyPhone: +49 62 24 143-0Fax:

30Instructions for Use V 1.4 – 10/2012After replacing the UVC lamp, its service life counter must be reset. To do so, press and hold the UVC button fo

31Leica CM1860 /CM1860UV – Cryostat6.2.2 Setting the automatic defrost time (cryochamber)The automatic defrost cycle takes place once every 24 hours.

32Instructions for Use V 1.4 – 10/20126.2.4 Activation of the Peltier element (option)The Peltier element provides additional cooling for the freezin

33Leica CM1860 /CM1860UV – Cryostat6. Operating the Instrument6.2.6 Manual defrosting of the cryochamberYou can activate manual defrosting of the

34Instructions for Use V 1.4 – 10/2012Advancing the specimen toward the knife• Startsfastorslowfeedtowardtheknife.TheLED(2) starts flashing

35Leica CM1860/CM1860 UV – Cryostat7. Daily Use of the Instrument7.1 Specimen freezing• Selectthesectioningtemperature(chambertemperature)a

36Instructions for Use V 1.4 – 10/20127. Daily Use of the Instrument7.2.1 Inserting the specimen discs into the specimen head7.2 Specimen discsFig

37Leica CM1860/CM1860 UV – Cryostat7. Daily Use of the Instrument7.3 Sectioning7.3.1 Blade holder CE 1 2 3When inserting the blade/knife holder ba

38Instructions for Use V 1.4 – 10/20127. Daily Use of the InstrumentInserting the blades into blade holder CEThe blade holder CE can be used either f

39Leica CM1860/CM1860 UV – Cryostat7. Daily Use of the Instrument4. Another option for removing the blade is to use the brush with magnet (12). T

4Instructions for Use V 1.4 – 10/2012Table of Contents1. Important Information ...

40Instructions for Use V 1.4 – 10/20127. Daily Use of the InstrumentFig. 34The safety gloves included in the stan-dard scope of delivery must be worn

41Leica CM1860/CM1860 UV – Cryostat7. Daily Use of the InstrumentClearance angle adjustmentThe harder the specimen is, the larger the clearance ang

42Instructions for Use V 1.4 – 10/20127. Daily Use of the InstrumentYou can adjust the height of the anti-roll guide system using the knurled nut (8)

43Leica CM1860/CM1860 UV – Cryostat7. Daily Use of the Instrument10986 7 Assembling the anti-roll guide system/replacing the anti-roll plate1. In

44Instructions for Use V 1.4 – 10/20127. Daily Use of the InstrumentFig. 407.3.3 Knife holder CN 1 2 3When inserting the blade/knife holder base, yo

45Leica CM1860/CM1860 UV – Cryostat7. Daily Use of the InstrumentInserting/removing the knifeThe height of resharpened knives must be adjusted usin

46Instructions for Use V 1.4 – 10/20127. Daily Use of the InstrumentFig. 44The knife guard (18) is fixed and integrated into the clamping jaws. The k

47Leica CM1860/CM1860 UV – CryostatKnife holder CN with anti-roll guide – Moving the clamping chucks23a443The clamping jaws are factory-installed in t

48Instructions for Use V 1.4 – 10/20127.3.4 Knife holder CN-Z with anti-roll guide3aFig. 48The height of resharpened knives must be adjusted using kn

49Leica CM1860/CM1860 UV – CryostatBlade holder CE1. To release the segment arc (2) from the base, use a 4 mm Allen key to loosen the screw over the

5Leica CM1860 /CM1860UV – Cryostat7. Daily Use of the Instrument ...

50Instructions for Use V 1.4 – 10/2012Trimming the specimenTake care when handling microtome knives and disposable blades. The cutting edge is extreme

51Leica CM1860/CM1860 UV – Cryostat7. Daily Use of the Instrument7.4 Temperature selection chart (in minus °C)The temperature values given in this

52Instructions for Use V 1.4 – 10/20127.5 DefrostingDefrosting the cryochamber actually means defrosting the evaporator to prevent excessive frost bui

53Leica CM1860 /CM1860UV – Cryostat7.5.3 Manual defrosting of the quick freeze shelfThe quick freeze shelf may become hot during defrosting! There-fo

54Instructions for Use V 1.4 – 10/20127.6.2 Shutdown for a longer periodIf you do not intend to use the instrument for several weeks you may turn it

55Leica CM1860/CM1860 UV – CryostatIf errors occur, they are output as error codes in the real-time display during normal operation with the format EO

56Instructions for Use V 1.4 – 10/20128.2 Temperature control buttonPossible causes and remedies:• Temperatureofthe direct surroundingsisconst

Leica CM1860/CM1860 UV – Cryostat578. Troubleshooting8.3 Possible sources of error, causes and remediesProblem Cause RemedyFrost on chamber walls

58Instructions for Use V 1.4 – 10/20128. TroubleshootingProblem Cause RemedySections not properly flattened despite correct temperature and correctly

Leica CM1860/CM1860 UV – Cryostat598. TroubleshootingProblem Cause RemedyCondensation on anti-roll plate and knife during cleaning- Brush, forceps

6Instructions for Use V 1.4 – 10/20121.1 Symbols in the text and their meaningsUseful tips, i.e. important information for the user, appear in gray

60Instructions for Use V 1.4 – 10/20128. TroubleshootingProblem Cause RemedyTissue adheres to the anti-roll guide.- Dried specimen.- Anti-roll plate

Leica CM1860/CM1860 UV – Cryostat618. TroubleshootingThe battery is exchanged during routine maintenance.If the customer has decided against a main

62Instructions for Use V 1.4 – 10/20129.1 Cleaning9. Cleaning, Disinfection, Maintenance12The catch tank collects the conden-sate that accumulates du

63Leica CM1860 /CM1860UV – Cryostat9.2 Spray disinfection with Leica Cryofect9. Cleaning, Disinfection, MaintenanceBasically, we recommend UV disi

64Instructions for Use V 1.4 – 10/20129. Cleaning, Disinfection, Maintenance9.3.1 General maintenance instructionsThe microtome is virtually mainten

65Leica CM1860 /CM1860UV – Cryostat9. Cleaning, Disinfection, MaintenanceFig. 576Detail 57a• Oilingtheslotcover(4, Fig. 55). To do so, first

66Instructions for Use V 1.4 – 10/20129.3.3 Replacing the UVC lampSwitch the instrument off and disconnect the power plug before replacing the fluore

67Leica CM1860/CM1860 UV – CryostatRemoval of the lampThe UVC lamp (3) is installed in front of the protection screen for chamber illumination (4). •

68Instructions for Use V 1.4 – 10/2012Uninstalling the lamp (cryochamber illumination)The fluorescent lamp (3) is installed hidden behind the protecti

69Leica CM1860/CM1860 UV – Cryostat10. Ordering Information, Components and Consumables10.1 Ordering informationBlade/knife holder and blade/knife

7Leica CM1860/CM1860UV – CryostatThe package contents are fragile and must be handled with care.Indicates the correct upright position of the package.

70Instructions for Use V 1.4 – 10/201210. Ordering Information, Components and ConsumablesEmbedding well bar, 4 x 18 mm...

71Leica CM1860/CM1860 UV – Cryostat10. Ordering Information, Components and ConsumablesTransfer block for specimen discs, large (holder for specime

72Instructions for Use V 1.4 – 10/2012Knife/blade holder base,for knife holder CN, blade holder CEOrder No. ...

73Leica CM1860/CM1860 UV – CryostatBlade holder CE-TC,for carbide metal disposable blades.Particularly suitable for sectioning harder materi-als, such

74Instructions for Use V 1.4 – 10/2012Fig. 72Knife support for knife holder CN,for short knivesOrder No. ...

75Leica CM1860/CM1860 UV – CryostatLeica high-profile disposable blades, Type 81880 x 14 x 0.317 mm1 pack of 50 ...

76Instructions for Use V 1.4 – 10/2012Knife, 16 cm carbide metal blade, profile cNote: including knife case 14 0213 11140Order No. ...

77Leica CM1860/CM1860 UV – CryostatFig. 81Fig. 82Fig. 83Knife, 12 cm length, profile d,for Leica CM1500 / CM1800Note: including knife case 14 0213 111

78Instructions for Use V 1.4 – 10/2012Fig. 84Cryoembedding system, completeconsisting of:- 3 embedding well bars with recesses, in three sizes: 18 m

79Leica CM1860/CM1860 UV – CryostatFig. 86Set of freezing griddle/heat extractor,elevatedconsisting of:- 1 forceps, epoxy-coated- 1 putty knife- E

8Instructions for Use V 1.4 – 10/20121. Important Information1.4 Instrument typeAll information contained in these Instructions for Use applies excl

80Instructions for Use V 1.4 – 10/2012Fig. 89Fig. 90Heat extractorOrder No. ... 14 0201 39125Container for sp

81Leica CM1860/CM1860 UV – CryostatFig. 92Blue O-ring,for color marking of the specimen disc20 and 30 mm (14 0477 40044)pack of 10.Order No. ...

82Instructions for Use V 1.4 – 10/2012Fig. 93Toolset for cryostats,consisting of:- 1 brush, narrow 14 0183 28642- 1 Leica brush with magnet 14 018

83Leica CM1860/CM1860 UV – CryostatEasy Dip, staining containerColor: whitepack of 6Order No. ... 14 0712 401

84Instructions for Use V 1.4 – 10/2012Specimen disc,20 mm ...Order No. 14 0370 0863625 mm ...

85Leica CM1860/CM1860 UV – Cryostat11 - Heat extractor, stationary (optional)2 - Peltier element (with 2 stations)3 - Freeze shelf, 8 positions4 -

86Instructions for Use V 1.4 – 10/2012Thermal block,for easy removal of frozen blocks from the speci-men disc (see also page 91)Order No. ...

87Leica CM1860/CM1860 UV – CryostatAdapter,for Miles Tissue Tek specimen discsOrder No. ... 14 0436 26747Fig.

88Instructions for Use V 1.4 – 10/2012Fig. 105Anti-roll guide system,for knife holder CN or CNZglass - 50 mmconsisting of:- Glass insert 50 mm- Meta

89Leica CM1860/CM1860 UV – CryostatGlass plate,glare-reducing, 70 mm wideSpare glass for anti-roll guide systems CE-BB:(14 0477 42491, 14 0477 42492,

9Leica CM1860/CM1860UV – Cryostat2. Safety2.1 Safety notesThese Instructions for Use include important in-structions and information related to th

90Instructions for Use V 1.4 – 10/2012Fig. 112Caution: Dangerous goods!Leica Cryofect,Low-temperature disinfectant,4 350 ml bottles.For temperatures d

91Leica CM1860/CM1860 UV – CryostatLapping device for knife,Profile c, 16 cm lengthOrder No. ... 14 0337 0868

92Instructions for Use V 1.4 – 10/2012Lapping stone (fine),Yellow with dark back, approx. 25 x 5.5 cmOrder No. ...

93Leica CM1860/CM1860 UV – CryostatScrew clamp,for fastening strop of type "Heidelberg"Order No. ...

94Instructions for Use V 1.4 – 10/201210.2 Mobile heat extractor – applicationSpecimen freezing with the freezing shelf can be accelerated by the ad-

95Leica CM1860 /CM1860UV – Cryostat11. Warranty and ServiceWarrantyLeica Biosystems Nussloch GmbH guarantees that the contractual product delivered

96Instructions for Use V 1.4 – 10/201212. Decontamination Certificate (Master)AThis equipment has not been in contact with unfixed biological samples

97Leica CM1860 /CM1860UV – Cryostat12. Decontamination Certificate (master)Important - to avoid refusal of shipment: Place one copy in the unit pri

98Instructions for Use V 1.4 – 10/2012Notes