Leica-biosystems RM2125 RTS User Manual

Leica RM2125 RTSRotary MicrotomeInstructions for UseLeica RM2125 RTSV2.1, English - 10/2012Order No. 14 0457 80101 RevBAlways keep this manual with t

10Instructions for Use, V 2.1 – 10/2012Knife guard on the knife holderEach knife holder is equipped with a tightly mounted knife guard (3, 5). This ma

11Leica RM2125 R TS3.1 Overview — instrument components3. Instrument Components and SpecificationsAdjusting knob for setting the section thicknessL

12Instructions for Use, V 2.1 – 10/20123. Instrument Components and Specifications3.2 Instrument specificationsThe Leica RM2125 RTS is a manually o

13Leica RM2125 R TS3.3 Technical DataInstallation site requirements Operating temperature range: 10 °C- 35 °CTemperature range during storage

14Instructions for Use, V 2.1 – 10/20124. Startup4.2 Standard delivery4.1 Installation site requirements- The instrument requires an installation

15Leica RM2125 RTS• Openthepackaging.• Takeoutallaccessories(1) and the Instruc-tions for Use.• RemovetheAllenkeyNo.8andlayitoffto

16Instructions for Use, V 2.1 – 10/20124. StartupThere are two versions of the specimen holder fixture – with and without specimen orientation. Spec

17Leica RM2125 RTS4. StartupThe specimen clamps (standard or universal cassette clamps) can also be fastened directly to the specimen holder fixtur

18Instructions for Use, V 2.1 – 10/20124. Startup• Releasetheclampinglever(20) by rotating it counterclockwise. (Position  = released)• Inser

19Leica RM2125 R TS5.1 Clamping the specimenAlwaysclampthespecimenblockBEFOREclampingtheknifeorthe blade.Lock the handwheel and cover the k

20Instructions for Use, V 2.1 – 10/20125. Operation• Theindexmarks(0°,5°and10°)foradjust-ment of the clearance angle (27) are located on th

21Leica RM2125 RTS5.4 Specimen retraction (specimen orientation)5. OperationFig. 13To prevent the knife or blade from touching the overlapped spec

22Instructions for Use, V 2.1 – 10/2012The specimen orientation allows for simple po-sition correction of the specimen surface when the specimen is cl

23Leica RM2125 RTSCoarse feed wheel (37)The coarse feed serves for a fast horizontal forwards movement of the specimen – towards the knife – and back

24Instructions for Use, V 2.1 – 10/20125. OperationTrimming the specimen with the coarse feed• Releasethehandwheellock.Todoso,pullthe handl

25Leica RM2125 RTSThe Leica RM2125 RTS is equipped with a me-chanical trimming function that is activated via the trimming lever (35). The trimming l

26Instructions for Use, V 2.1 – 10/20125.7 SectioningAlways rotate the handwheel at a uniform speed. The rotation speed of the handwheel must be adapt

27Leica RM2125 RTS5. Operation5.9.1 Standard specimen clamp (optional)The standard specimen clamp is suitable for holding specimens of 40 x 40 mm.

28Instructions for Use, V 2.1 – 10/20125. Operation5.9.2 Vee insert (optional) The vee insert (44) is mounted in the hole pro-vided in the lower mo

29Leica RM2125 RTS5. OperationClamping of foil pieces• Movethemovablejaw(48) to the right as re-quired by turning the setscrew with an Allen k

3Leica RM2125 RTSThe information, numerical data, notes and val-ue judgments contained in this manual repre-sent the current state of scientific know

30Instructions for Use, V 2.1 – 10/2012• Toopenthejaws(56) and (57), slightly loos-en the three screws (55) with an Allen key No. 4 (49).• Inser

31Leica RM2125 RTSPriortosectioning,laboratoryperson-nel MUST check that the cassette is seated securely in the universal cas-sette clamp.• Push

32Instructions for Use, V 2.1 – 10/2012• Tomounttherequiredinsert(62, 63, 64) turn the clamping ring (66) counterclockwise and remove it.• Plac

33Leica RM2125 RTSKnife holder base without lateral movementThe one-piece knife holder base without lateral movement (21.1) can be moved only forward

34Instructions for Use, V 2.1 – 10/20125. Operation5.9.8 KnifeholderN/NZTheknifeholdersNandNZareappropriateforstandardsteelandtungste

35Leica RM2125 RTSInserting the knife• Rotatetheknurlednuts(48) on the right and left of the knife holder forward in opposite directions, loweri

36Instructions for Use, V 2.1 – 10/2012Priortoinsertingtheblade,bothknifeholder and knife holder base must have been installed on the instrumen

37Leica RM2125 RTSInserting the bladePriortoinsertingtheblade,bothknifeholder and knife holder base must have been installed on the instrument

38Instructions for Use, V 2.1 – 10/2012After each uninstallation or replacement, make sure the pressure plate sits correctly. If necessary, readjust i

39Leica RM2125 RTSAdjusting the front pressure plate• Bringthepressureplate(86) into position, insert the clamp-ing lever (89), and use the clam

4Instructions for Use, V 2.1 – 10/2012Table of Contents1. Important Information ...

40Instructions for Use, V 2.1 – 10/20125. OperationFig. 345.9.11 Overview – accessoriesKnife holder Efor bladesKnife holder Nfor steel and carbide m

41Leica RM2125 R TS6. Cleaning and Maintenance6.1 Cleaning the instrumentAlwaysremovetheknife/bladebeforedetachingtheknifeholderfromthe

42Instructions for Use, V 2.1 – 10/20126. Cleaning and MaintenanceKnife holder EFig. 35Take the knife holder apart for cleaning. The front pressure

43Leica RM2125 RTSUniversal cassette clamp• Detachcassetteclamp(13) for a thorough cleaning, removing all paraffin residues.• Forcleaning,d

44Instructions for Use, V 2.1 – 10/20126.2 Maintenance instructionsOnlyauthorizedandqualifiedservicepersonnelmayaccesstheinternalcomponents

45Leica RM2125 R TS7. Optional accessoriesKnife holder base, non-orientable, silver ...

46Instructions for Use, V 2.1 – 10/2012Knife holder base, non-orientableSilver for knife holders N, NZ, E, and E-TCOrder No. ...

47Leica RM2125 RTSKnifeholderNZ,silverfor holding conventional and carbide metal knives up to 16 cm in length, knife pressure plate for extreme st

48Instructions for Use, V 2.1 – 10/2012Knife holder E,for high-profile microtome blades (80 x 14 x 0.317 mm),RM2125 RTS, silver, quick clamp system c

49Leica RM2125 RTSWater bath assemblyRM2125 RTS, for knife holder E for high-profile and low-profile disposable bladesOrder No. ...

5Leica RM2125 R TSTable of Contents5.9.6 Round specimen holder (optional) ...

50Instructions for Use, V 2.1 – 10/2012Disposable blades Leica TC-65Leica TC-65 microtome, disposable blade system for sectioning hard specimen mate-r

51Leica RM2125 RTS7. Optional accessoriesKnife 16 cm, profile d - steelKnife, 16 cm long, profile dNote: Knife case 14 0213 11140 includedOrder No.

52Instructions for Use, V 2.1 – 10/2012Specimen holder fixture, non-orientableRM2125 RTS, silverTo modify your microtome with these accessories, plea

53Leica RM2125 RTSUniversal cassette clampwith adapterRM2125 RTS, silverFor use with standard cassettes with dimen-sions that are 39.8 x 28mm at mini

54Instructions for Use, V 2.1 – 10/2012Roundspecimenholderwith adapter,without clamping rings, silverOrder No. ...

55Leica RM2125 RTSClamping ringfor round specimen holder15 mm diameterOrder No. ... 14 0356 092007. Option

56Instructions for Use, V 2.1 – 10/20127. Optional accessoriesDust coverOrder No. ... 14 0212 30350Fig. 66S

57Leica RM2125 R T SIn the following table there is a list of the most common problems which can arise while working with the instrument, along with

58Instructions for Use V 2.1 – 10/2012Problem Corrective actionPossiblecause8.2 Instrument malfunctions1. There is no further feed motion and thus n

59Leica RM2125 R TS9. Warranty and ServiceWarrantyLeica Biosystems Nussloch GmbH guarantees that the contractual prod-uct delivered has been subject

6Instructions for Use, V 2.1 – 10/20121. Important InformationNotes, i.e. important information for the user, are highlighted in gray and marked b

60Instructions for Use, V 2.1 – 10/2012Notes

7Leica RM2125 RTSThe safety and caution notes in this chapter must be observed at all times. Be sure to read these notes even if you are already fami

8Instructions for Use, V 2.1 – 10/20122. SafetyWarnings – Markings on the instrument itself• Safetynotesontheinstrumentitselfmarkedwithawa

9Leica RM2125 RTSWarnings – Maintenance and Cleaning• Onlyauthorizedandqualifiedservicepersonnelmayaccesstheinternalcomponentsoftheinst