Leica-biosystems CM1100 User Manual

Instructions for useLeica CM1100 V1.3 RevB English – 09/2012Order-No.: 14 0469 80101, RevBAlways keep this manual near the instrument!Read carefully p

10 Instructions for use V1.3 RevB – 09/20125. General description5.2 Product descriptionThe Leica CM1100 is a portable compact cryostat for rapid free

11Leica CM1100 – Cryostat6. Unpacking and installation6.1 UnpackingCheck the transport crate for external dam-age on arrival of the shipment.If damage

12 Instructions for use V1.3 RevB – 09/20126.2 Site requirementsDo not operate in rooms with explosion haz-ard!To ensure an adequate cooling capacity,

13Leica CM1100 – Cryostat7.2 Installing the accessories• Remove the lid.• To release, turn the clamping lever (1) of the micro-tome base plate counter

14 Instructions for use V1.3 RevB – 09/2012The control unit has a 3-digit display for actual and settemperature and defrost time, with additional LEDs

15Leica CM1100 – Cryostat7.4.4 Starting and terminating a manual defrostcycle• To activate manual defrosting, press 'P' and the'Arrow-u

16 Instructions for use V1.3 RevB – 09/2012AdrenalsBone marrowBrainBladderBreast- fattyBreast - little fatCartilageCervixFatHeart and vesselIntestineK

17Leica CM1100 – CryostatSpecimen discs (1) are available in different sizes of20 and 25 mm in diameter. The design of the surfaceensures firm contact

18 Instructions for use V1.3 RevB – 09/2012Fig. 18.18.2 Knife holder CEThe knife holder CE is equipped with a pres-sure plate for low profile blades o

19Leica CM1100 – Cryostat8. Daily operation8.2.4 Inserting the bladeTake care when handling disposable blades.The cutting edge is extremely sharp and

20 Instructions for use V1.3 RevB – 09/20128. Daily operation8.2.5 Lateral displacementIf the sectioning results start lacking quality, the knifeholde

21Leica CM1100 – CryostatI wrong: anti-roll guide positioned too lowII wrong: anti-roll guide positioned too highIII right: anti-roll guide correctly

22 Instructions for use V1.3 RevB – 09/20128.4 Trimming• Remove the anti-roll guide from the cutting edge.• Unlock the handwheel.Always observe the sp

23Leica CM1100 – Cryostat8. Daily operation8.5 Sectioning8.6 Section transferTake care when removing the section - thecutting edge is exposed!Section

24 Instructions for use V1.3 RevB – 09/20128. Daily operation8.7 DefrostingThe Leica CM 1100 provides both programmable auto-matic and manual defrosti

Leica CM1100 – Cryostat259. Trouble shootingProblem Possible causes RemediesFrost on chamber walls and microtomewhile cryochamber lid not in placeFros

26Instruction manual, V1.2 – 06/20069. Trouble shooting- Refreeze specimen onto the disc.- Check disc clamping.- Check clamping.- Refreeze specimen on

Leica CM1100 – Cryostat279. Trouble shootingFlattened sections curl up when anti-roll plate is picked upSections tearInconsistent or insufficient spec

28 Instructions for use V1.3 RevB – 09/201210. Cleaning and disinfection• Remove frozen section waste from the cold cry-ostat.• Remove the waste trays

29Leica CM1100 – CryostatIf increased frost buildup occurs on the evapo-rator, start a manual defrost cycle.If increased frost buildup occurs on the m

3Leica CM1100 – Cryostat1. Important informationLeica Biosystems Nussloch GmbHHeidelberger Str. 17-19D-69226 NusslochGermanyTelephone: 06224/143-0Tele

30 Instructions for use V1.3 RevB – 09/201210.4 Reassembly of the microtomeThe microtome must be completely dry beforereinstallation. Humidity inside

31Leica CM1100 – Cryostat11.2 Fuse replacementOnly use a fuse of the same specification! Forthe required value, please refer to Chapter 4.'Techn

32 Instructions for use V1.3 RevB – 09/2012The thermal block (8) facilitates the removal of the fro-zen specimen from the specimen disc.Keep the therm

33Leica CM1100 – Cryostat13. Warranty and serviceWarrantyLeica Biosystems Nussloch GmbH guarantees that the contractual product deliveredhas been subj

34 Instructions for use V1.3 RevB – 09/201214. Decontamination Certificate (master)Important - to avoid refusal of shipment:Place one copy in the unit

35Leica CM1100 – Cryostat15. Information for the People´s Republic of China

36 Instructions for use V1.3 RevB – 09/2012Notice

4 Instructions for use V1.3 RevB – 09/20122. Table of contents1. Important information ...

5Leica CM1100 – Cryostat3. Safety instructions for handling the instrument3.1 Safety featuresThe instrument design incorporates the followingsafety fe

6 Instructions for use V1.3 RevB – 09/2012Connection to mains power• Please refer to the 'Technical data'!• The instrument must be connected

7Leica CM1100 – Cryostat3. Safety instructions for handling the instrumentSectioning• Take care when handling disposableblades.The cutting edge is ext

8 Instructions for use V1.3 RevB – 09/20124. Technical dataOperating temperature range: 18 °C to 35 °C.All specifications related to temperature are v

9Leica CM1100 – Cryostat5.1 Leica CM 1100 Overview5. General descriptionCryochamberlidCarryinghandlesControl unitHandwheelwith retractablecountersunk