Leica-biosystems RM2235 User Manual

Instructions for UseRM2235Rotary MicrotomeLeica RM2235 V 1.8 RevC, English 09/2013Order No.: 14 0500 80101 RevCAlways keep this manual with the instru

8Instructions for Use V 1.8 RevC – 09/2013• Tolockthehandwheel,pressthelever(5) outwards and continue to turn the handwheel slowly until it lo

9Leica RM22352. SafetyKnife guard on the knife holderEach knife holder is equipped with a tightly mounted knife guard (8, 9). This makes it possibl

10Instructions for Use V 1.8 RevC – 09/20133.1 Overview — instrument components3. Instrument Components and SpecificationsAdjusting knob for setting

11Leica RM22353. Instrument Components and Specifications3.2 Instrument specificationsBasic instrument with mechanical trim function, lateral coar

12Instructions for Use V 1.8 RevC – 09/20133.3 Technical DataGeneral Approvals: The instrument-specific marks are located on the rear panel of the

13Leica RM22354. Instrument Setup4.1 Standard deliveryThe Leica RM2235 standard delivery includes: 1 Leica RM2235 basic instrument 1 handwheel,

14Instructions for Use V 1.8 RevC – 09/20134.3 Unpacking and installationFig. 6Fig. 712344445• Loosen and unscrew the six upper screws (2).• Remove

15Leica RM2235654.3 Unpacking and installation (cont.)Fig. 8789• Takeoutthefixingmodule(5). To do so, hold it by the top edge of the module and

16Instructions for Use V 1.8 RevC – 09/20134. Instrument SetupThe feather key (4) is loosely placed in the handwheel shaft (1) and fixed in place wit

17Leica RM22354. Instrument SetupThere are two versions of the specimen holder fixture, one with and one without specimen ori-entation, which are

18Instructions for Use V 1.8 RevC – 09/20134. Instrument SetupSetting up the knife holder base• Releasetheclampinglever(50) by rotating it count

19Leica RM22355. Operation5.1 Operating elements and their functionsFig. 13 The section thickness is set by turning the ad-justing knob (33) at th

20Instructions for Use V 1.8 RevC – 09/20135. Operation5.1.3 Specimen retractionThe specimen retraction serves for protecting the knife and the speci

21Leica RM22355. Operation5.1.5 Specimen holder with precision orientationOrienting the specimenFig. 17Display of the zero positionFor better disp

22Instructions for Use V 1.8 RevC – 09/20135. Operation• Theindexmarks(0°,5°and10°)foradjustmentof the clearance angle (59.1) are located on

23Leica RM22353334Important!Never turn the screw more than 1/2 turn at a time.5.3 Fine adjustment of the force balanceIf another accessory of a diffe

24Instructions for Use V 1.8 RevC – 09/20135. Operation5.4 Clamping the specimen• Rotatethehandwheeluntilthespecimenclampisintheuppermost

25Leica RM22355.6 Sectioning5. OperationAlways turn the handwheel evenly in clockwise direction; other-wise, the brake will not work properly. The

26Instructions for Use V 1.8 RevC – 09/20135.7 Changing the specimen or interrupting sectioning5.8 Finishing the daily routine• Movethespeciment

27Leica RM22356.1 Assembly for fixture for specimen clamps6.1.1 Rigid fixture for specimen clamps• Screw the rigid fixture for specimen clamps (4)

1Leica RM2235The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge a

28Instructions for Use V 1.8 RevC – 09/20136. Optional Accessories6.1.3 Fine-directional fixture for specimen clamps• Beforethefine-directional

29Leica RM22356.1.4 Quick clamping systemIt is used as specimen holder for use with the fine-directional fixture for specimen clamps with zero point

30Instructions for Use V 1.8 RevC – 09/20136. Optional Accessories6.2.1 Standard specimen clampThe standard specimen clamp is available in two sizes

31Leica RM2235The vee insert (70) is mounted in the hole pro-vided in the lower movable jaw of the standard specimen clamp. This makes it possible to

32Instructions for Use V 1.8 RevC – 09/20136. Optional AccessoriesClamping of foil pieces• Movethemovablejaw(74) to the right as re-quired by tu

33Leica RM2235• Pushthelever(60) forwards.• Mountthecassette(65) horizontally or verti-cally as required.• Toclampthecassette,releasethe

34Instructions for Use V 1.8 RevC – 09/20136.2.5 Super mega-cassette clampFig. 3012345Assembly of the super mega-cassette clamp The super mega casse

35Leica RM2235• Tomounttherequiredinsert(89.1-3) turn the clamping ring (90) counterclockwise and remove it.• Placetherequiredinsertinto

36Instructions for Use V 1.8 RevC – 09/20136. Optional Accessories6.3 Knife holder base and knife holderRepositioning the knife holder baseThe one-p

37Leica RM22356. Optional AccessoriesInserting the blade, knife holder E and E-TC• Foldknifeguard(9) downward.• Toinserttheblade,flipthe

2Instructions for Use V 1.8 RevC – 09/2013Table of Contents1. Important Information ...

38Instructions for Use V 1.8 RevC – 09/20136. Optional AccessoriesLateral displacement (only for knife holder E)The lateral displacement feature of t

39Leica RM22356. Optional Accessories Knife holder E with a water trough for low-profile bladesFig. 37The knife holder E with a water trough is on

40Instructions for Use V 1.8 RevC – 09/20136.3.3 Knife holder N/NZFig. 3984647494849Enlarged detail: Knife inserted and height-adjusted396. Optional

41Leica RM22356. Optional AccessoriesInserting the knife• Rotatetheknurlednuts(48) on the right and left of the knife holder forward in opposi

42Instructions for Use V 1.8 RevC – 09/20136. Optional Accessories• Pushthesectionwastetray(18) from the front to the microtome base plate (53)

43Leica RM22356. Optional Accessories6.7 Freezer pack989998The freezer pack consists of the freezer plate (98) and the insulation jacket (99). It

44Instructions for Use V 1.8 RevC – 09/20136.8 Universal microscope carrier 6. Optional Accessories1 - Baseplate with drilling (a)2 - Vertical colu

45Leica RM22352. Attach vertical column• Insertthecapscrew(b) into the hole of the baseplate from below. Place the lock washer (c) on the cap sc

46Instructions for Use V 1.8 RevC – 09/20136. Optional Accessories6.9 Magnifying lens Fig. 50Fig. 52Fig. 51• Openthescrew(3) on the horizontal a

47Leica RM2235Knife holder base, non-orientableSilver for knife holders N, NZ, E, and E-TCOrder No. ... 14 05

3Leica RM2235Table of Contents6.1.3 Fine-directional fixture for specimen clamps ...

48Instructions for Use V 1.8 RevC – 09/20136. Optional AccessoriesKnife holder E, for low-profile disposable blades, (80 x 8 x 0.25 mm), silver, for

49Leica RM2235Knife holder E-TC, for carbide metal disposable blades TC-65, silverQuick clamp system, rustproof clamping plate made of stainless steel

50Instructions for Use V 1.8 RevC – 09/2013Disposable blades Leica TC-65Leica TC-65 microtome, disposable blade system for sectioning hard specimen ma

51Leica RM2235Knife, 16 cm, profile d, tungsten carbideKnife, 16 cm long, tungsten carbide, profile dNote: Knife case 14 0213 11140 includedOrder No.

52Instructions for Use V 1.8 RevC – 09/2013Universal cassette clampwith adapterRM2125 RTS, silverFor use with standard cassettes with dimensions that

53Leica RM2235specimen holder fixture, directionalsilverFor holding the standard clamp,universal cassette clamp,round specimen holder and EM specimen

54Instructions for Use V 1.8 RevC – 09/2013Quick clamping system, for specimen holder for use with the fine-direc-tional fixture for specimen clamps w

55Leica RM2235Foil clamp Type I for standard specimen clamp, blackMaximum specimen size: 25 x 13 mmOrder No. ...

56Instructions for Use V 1.8 RevC – 09/2013EM universal specimen holder,For samples with a 8.5 mm diameterOrder No. ...

57Leica RM2235Electrically cooled universal cassette clamp with adapter for the Leica RM2200 series rotary mi-crotomes. For use with standard cassette

4Instructions for Use V 1.8 RevC – 09/2013Symbols in the text and their meanings1. Important Information(5)Designated useThe Leica RM2235 is a manual

58Instructions for Use V 1.8 RevC – 09/2013Magnifier,For assembly on microscope carriers (14 0502 40580), 2x magnificationNote:Magnifier includes an

59Leica RM2235Leica CLS 100, cold light source,with power adapter – USA 100-120 V / 50-60 HzOrder No. ... 14

60Instructions for Use V 1.8 RevC – 09/2013Tray,for rotary microtome series Leica RM2200Order No. ... 14 0502

61Leica RM2235Brush, "Leica" with magnet for blade removal tool for knife holder E.Order No. ... 14

62Instructions for Use V 1.8 RevC – 09/20136. Optional Accessories6.11 Ordering informationKnife holder-base rigid, silver ...

63Leica RM22356. Optional AccessoriesCold light sources Leica CLS 100X, 100-120 V/50-60 Hz ...

64Instructions for Use V 1.8 RevC – 09/20137. TroubleshootingIn the following table there is a list of the most common problems which can arise while

65Leica RM22357. TroubleshootingProblem Possible cause Corrective action• When using cassettes, particu-larly thin-walled cassettes, from a compan

66Instructions for Use V 1.8 RevC – 09/2013Problem Possible cause Corrective action4. Noises during sectioning The knife "sings" when secti

67Leica RM2235Always remove the knife / blade before detaching the knife holder from the instrument.Always put the knives back into the knife case whe

5Leica RM22352. Safety2.1 Safety notesThese Instructions for Use include important information related to the operating safety and maintenance of

68Instructions for Use V 1.8 RevC – 09/2013• Laytheremovedpartsonanabsorbentclothintothe drying chamber (up to a max. of 65°C) and let the p

69Leica RM2235Universal cassette clamp• Detachcassetteclamp(13) for a thorough cleaning, removing all paraffin residues.• Forcleaning,donotu

70Instructions for Use V 1.8 RevC – 09/20138. Cleaning and Maintenance97 88731110Knife holder E:• Clamping lever (11) for the lateral displace-men

71Leica RM22359. Warranty and ServiceWarrantyLeica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to

72Instructions for Use V 1.8 RevC – 09/201310. Decontamination Certificate (Master)Leica Biosystems Nussloch GmbHHeidelberger Str. 17-1969226 Nussloc

73Leica RM223510. Decontamination Certificate (Master)Leica Biosystems Nussloch GmbHHeidelberger Str. 17-1969226 Nussloch, GermanyPhone: ++49 62

74Instructions for Use V 1.8 RevC – 09/2013Notes

www.LeicaBiosystems.comLeica Biosystems Nussloch GmbHHeidelberger Straße 17-19D- 69226 NusslochPhone: +49 - (0) 6224 - 143 0Fax: +49 - (0) 6224 - 143

6Instructions for Use V 1.8 RevC – 09/2013Safety instructions — working with the instrumentWarnings — Safety notes on the instrument itselfWarnings —

7Leica RM22352. SafetySafety instructions — working with the instrument• Always lock the handwheel before cleaning!• Do not use any solvents cont