Leica-biosystems CM1850 UV User Manual

Instructions for UseLeica CM1850, V2.6, English – 10/2012Order No. 14 0471 85101 RevCAlways keep this manual near the instrument!Read carefully prior

10Instructions for use V 2.6 – 10/2012• Never leave knives and knife holders with a knife/blade mounted lyingaround!• Do not place a knife on a table

11Leica CM1850 – CryostatExtended skin contact with cold parts of the instrument may cause frostbite!• The microtome must be entirely dry before reins

12Instructions for use V 2.6 – 10/2012Type CM1850 CM1850 CM1850-08 CM1850-01Mark of conformity CUL - - VDENominal voltage (±10%) 120 V AC 220 V AC 230

13Leica CM 1850 – CryostatPlease refer to section 5.2 ‘Site require-ments‘!3. Technical dataAll CM 1850 types require the following fuses:F1: T0,25 A

14Instructions for use V 2.6 – 10/20124.1 Site requirementsDo not operate in rooms with explosion hazard!To ensure an ad-equate cooling capacity, the

15Leica CM 1850 – Cryostat4.3 Standard deliveryIt gives following variations: - CM1850 with Retraction, different voltages- CM1850 without Retraction

16Instructions for use V 2.6 – 10/20124.4 Handwheel assembly• Insert the pin (1) of the handwheel shaft in tothe hole (2) of the handwheel.• Mount the

17Leica CM1850 – Cryostat5.1 Connection to mains powerDuring the start-up of the compressor the nominal voltage must notdrop below the values specifie

18Instructions for use V 2.6 – 10/20124161518192021813101291113145.3 Leica CM1850 - Overview5. Setup1317Fig. 6

19Leica CM1850 – Cryostat1 Cryostat CM18502 Waste container3 Control panel 14 Control panel 25 Storage shelf, left6 Storage shelf, right7 Automatic ma

20Instructions for use V 2.6 – 10/20125.4 Mains switch and automatic mains fuseThe automatic mains fuse is used as mains switch.To turn the automatic

21Leica CM1850 – Cryostat6. Control panel operation6.1 Control panel 1Function keysLamp buttonON/OFF switch for cryochamber illumination.Manual defros

22Instructions for use V 2.6 – 10/20126. Control panel operation6.2.2 Setting the automatic defrost time (cryochamber)The automatic defrost cycle tak

23Leica CM1850 – Cryostat6.2.4 Activation of the Peltier elementThe Peltier element is used for cooling the quick-freeze stations. Upon acti-vation of

24Instructions for use V 2.6 – 10/20126.2.6 Manual defrosting of the cryochamberThe manual defrosting of the cryochamber is activated by subsequentlyp

25Leica CM1850 – Cryostat6.4 Control panel 2 - Electric coarse feedMove the specimen away from the knife• Press to start a rapid return travel of the

26Instructions for use V 2.6 – 10/20127. Daily use of the instrument7.1 Specimen freezing• Select the appropriate sectioning temperature (cryochamber

27Leica CM 1850 – Cryostat7. Daily use of the instrument7.2 Specimen discs 7.2.1 Inserting the specimen discs in thespecimen head• Lock the handle of

28Instructions for use V 2.6 – 10/20127.3 SectioningAll necessary adjustments on the knife holder and anti-roll guideare described in the separate ins

29Leica CM1850 – Cryostat7.4 Temperaturew selection chart (in minus °C)Tissue -10°C – -15°C -15°C – -25°C -25°C – -35°CAdrenal ❄❄Bone marrow ❄Brain ❄

3Leica CM 1850 – CryostatLeica Biosystems Nussloch GmbHHeidelberger Str. 17 - 19D-69226 NusslochGermanyPhone: +49 (62 24) 143-0Fax: +49 (62 24) 143

30Instructions for use V 2.6 – 10/20127.5 DefrostingDefrosting the cryochamber actually means defrosting the evaporator toprevent excessive frost buil

31Leica CM1850 – Cryostat7.5.3 Manual defrosting of the quick freeze shelfThe quick freeze shelf may become hot during defrosting!Therefore, do not to

32Instructions for use V 2.6 – 10/20127.6.2 Shutdown for a longer periodIf you do not intend to use the instrument for several weeks youmay turn it of

33Leica CM 1850 – Cryostat8.1 Error messages in the displayError messages are displayed on the clock panel as follows: EO: XX. Thefollowing error mess

34Instructions for use V 2.6 – 10/20128. Troubleshooting8.3 Possible causes and remediesFrost on chamber walls and mi-crotomeIce formation on the bott

35Leica CM1850 – CryostatProblem Cause RemedySections not properly flatteneddespite correct temperature andcorrectly aligned anti-roll plateSections c

36Instructions for use V 2.6 – 10/2012Problem Cause Remedy- Knife profile inappropriate forthe specimen to be cut.- Incorrect clearance angle.- Brush,

37Leica CM1850 – CryostatProblem Cause Remedy8. TroubleshootingFat on the corner or edge of theanti-roll plate.- Rusty knife/blade.- Static electricit

38Instructions for use V 2.6 – 10/2012No or insufficient refrigerationScraping noise at the microtome- Compressor defective.- Leak in the cooling syst

39Leica CM 1850 – Cryostat9.1 Cleaning• Remove frozen section waste from the cryostat with a cold brush everyday.• Remove the section waste tray for e

4Instructions for use V 2.6 – 10/2012Table of contents1. Important Information ...

40Instructions for use V 2.6 – 10/20129.2 Spray disinfection with Leica CryofectFor easy-to-use spray disinfection we recommend Leica Cryofect.The cry

41Leica CM 1850 – Cryostat9.3 Maintenance 9.3.1 General maintenanceThe microtome is virtually maintenance-free. Toensure a smooth operation of the ins

42Instructions for use V 2.6 – 10/2012the lowest position and apply some drops of cryostat oil on to the slotcover; distribute the applied oil by turn

43Leica CM 1850 – Cryostat9. Cleaning, disinfection, maintenance• Loosen the screws (3) with an Allen key (4 mm).Do not loosen the screws (7) for remo

44Instructions for use V 2.6 – 10/2012The microtome cover may be removedto expeditethorough drying of the mi-crotome in an oven. Note:Place the microt

45Leica CM 1850 – CryostatEnsure that the microtome is completely dry before reinstallation.Humidity inside will condense and freeze and thus cause ma

46Instructions for use V 2.6 – 10/20129. Cleaning, disinfection, maintenance9.3.5 Replacement of the fusesTurn the instrument off with the automatic m

47Leica CM 1850 – Cryostat9. Cleaning, disinfection, maintenance• Turn the instrument off with the automaticmains fuse.• Pull the mains plug.• Slightl

48Instructions for use V 2.6 – 10/2012Knife holder base ...

49Leica CM1850 – CryostatAntistatic kit for knife holder CE, low profile... 14 0800 377

5Leica CM1850 – CryostatTable of contents7. Daily use of the instrument ...

50Instructions for use V 2.6 – 10/201210.2 Optional accessories10.2.1 Mobile heat extractorSpecimen freezing with the freezing shelf can be accelerate

51Leica CM1850 – Cryostat11. Warranty and serviceWarrantyLeica Biosystems Nussloch GmbH guarantees that the contractual productdelivered has been subj

52Instructions for use V 2.6 – 10/201212. Decontamination Certificate (master)DescriptionName/Model Fabr. No.KAT No. QuantityTick Box A if applicable.

53Leica CM1850 – Cryostat12. Decontamination Certificate (master)Important - to avoid refusal of shipment:Place one copy in the unit prior to packagin

54Instructions for use V 2.6 – 10/2012

6Instructions for use V 2.6 – 10/20121.1 Symbols used in this manual andtheir meaningWarningsappear in a grey box and are markedby a warning triangle

7Leica CM1850 – Cryostat2.1 Safety featuresThe instrument and its accessory equipment incorporate the following safetyfeatures: safety handwheel and

8Instructions for use V 2.6 – 10/20122.1.2 Knife guardFor every manipulation in the cryochamber, or upon changing aspecimen when the knife or disposab

9Leica CM1850 – Cryostat2. Safety2.3 Operating conditionsTransport and installation• After transporting do not turn the instrument on for a minimum of